Clone

Target
IDP91766  
Clone Suffix
1
Experiment Start Date
Jul 22, 2011  
Experiment End Date
Aug 01, 2011  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
162  
Reference Sequence Used
MSEIVIRHAE TRDYEAIRQI HAQPEVYCNT LQVPHPSDHM WQERLADRPG IKQLVACIDG DVVGHLTIDV QQRPRRSHVA DFGICVDSRW KNRGVASALM REMIEMCDNW LRVDRIELTV FVDNAPAIKV YKKYGFEIEG TGKKYALRNG EYVDAYYMAR VK  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAGTGAGATAGTAATACGCC  
PCR Backward Primer
TTATCCACTTCCAATGTTACTTCACCCGCGCCATAT  
Shipped To Lab
ANL  
Shipped Date
Sep 29, 2011  
Inserted
Oct 04, 2011  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2011-08-02 2011-08-02 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay
2011-12-13 2011-12-15 Min Zhou Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 1 mM IPTG production