Clone

Target

IDP00347  

Clone Suffix

2 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Sep 04, 2008  

Experiment End Date

Sep 05, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

266  

Reference Sequence Used

MKQAIGFIDS GVGGLTVVRE VLKQLPHEQV YYLGDTARCP YGPRDKEEVA KFTWEMTNFL VDRGIKMLVI ACNTATAAAL YDIREKLDIP VIGVIQPGSR AALKATRNNK IGVLGTLGTV ESMAYPTALK GLNRRVEVDS LACPKFVSVV ESGEYKSAIA KKVVAESLLP LKSTKIDTVI LGCTHYPLLK PIIENFMGDG VAVINSGEET ASEVSALLDY HNLLDATDEE IEHRFFTTGS TQIFKDIAKD WLNMPDMTVE HIKLGK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MKQAIGFIDS GVGGLTVVRE VLKQLPHEQV YYLGDTARCP YGPRDKEEVA KFTWEMTNFL VDRGIKMLVI ACNTATAAAL YDIREKLDIP VIGVIQPGSR AALKATRNNK IGVLGTLGTV ESMAYPTALK GLNRRVEVDS LACPKFVSVV ESGEYKSAIA KKVVAESLLP LKSTKIDTVI LGCTHYPLLK PIIENFMGDG VAVINSGEET ASEVSALLDY HNLLDATDEE IEHRFFTTGS TQIFKDIAKD WLNMPDMTVE HIKLGK  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAACAAGCTATTGGCTTTAT  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTTCCTAATTTAATATGTTCTA  

Shipped To Lab

UT  

Shipped Date

Oct 09, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008