Clone

Target

IDP00155  

Clone Suffix

9 The clone is not available in BEIR.

Sequence Comments

 

Experiment Start Date

Nov 02, 2011  

Experiment End Date

Nov 02, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

opMCSG28  

Sequence Start

1  

Sequence End

575  

Reference Sequence Used

MPINKLRKRL GDLLVEEGIV SEAQLEQALN AQKNTGRRLG DTLISLGFLS ETQLLNFLAQ QLSLPVIDLS RAHVDIDAVP LLPEVHARRL RALVIGRSGD TLRIAMSDPA DLFAQEALLN QLPDYGFEFV IAPEKQLVDG FDRYYRRTKE IVSFAEQLHA EHKTNDSFDF EITDSDSDEV TVVKLINSLF EDAIQVGASD IHIEPDANVL RLRQRIDGVL HETLLHEVNI ASALVLRLKL MANLDISEKR LPQDGRFNIR VKGQSVDIRM STMPIQHGES VVMRLLNQSA GVRKLEESGI PPHLLLRLRH QLKRPHGMIL VTGPTGSGKT TTLYGALNEL NTSGKKIITA EDPVEYRISR INQVQVNPKI NLDFSTLLRT FLRQDPDIIL VGEMRDQETV EIGLRAALTG HLVLSTLHTN DAVDSALRLI DMGAPGYLVA SAVRAVVAQR LVRKVCPDCS GHDEVDEARR QWLVTRFPNQ AAAKFTRGRG CQNCNLTGYR GRIGVFEMLE LDQPMMDCLR ANDAVAFSKA ARSNTDYKPL LASAMELALQ GIVSLDEVMS LGEGDSSGLV EPIYL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

LTLRRSLSHA N**TA*TAW* LAG*RGDCER SAA*TSAQCP EKYRTPLRGY LNFAWLFKRN PVAELFGAAI EFASDRSEPC PCRY*CCSFT A*SSCPPFAC AGDRPQW*YA AHSHE*SCGF VCSRSLAQPV A*LWF*VCHR P*EAVGRWL* SLLSPHQRNC LVCRAIACRT QNQ**F*F*D HRFR*R*SDG C*TDQLAV*R CDSSGSLGYT Y*AGCQRTAF APTDRWGIA* NLAP*SEYRL SLSAALKING ESGYFRKTPS ARWPL*HPCK RTIRRYPDVN HAYPAR*IGG DAFAQPICRG *KARRVRYSA SFIAAFAPSV ETPSWHDSGD WAYRLGENHH LVWRTQRAQY VRQKNHYCGR SGGIPDFTDQ SGASEPENQS RFLDVTAHFL APRPRYYFGR *DA*PRDGRN WPARCINRSL SLKYSAHQ*C RR*RAAFD*Y GGARLSGCQC GTCRGCAAFS AKSLSRL*WT **GG*SASPM ACDAFP*SSC RQIHSWTRLP EL*LNRLSRP DRCI*NA*AG SADDGLLAGK *CCGLL*SRT Q*HRL*TATG FGDGVSATRH RQS**SDVAG *R*FLWFG*T DLSGGEPVLP  

PCR Forward Primer

GTCTCTCCCATGCCAATTAATAAACTGCGTAA  

PCR Backward Primer

GGTTCTCCCCACGCAGATAAATCGGTTCAACCAAA  

Shipped To Lab

ANL  

Shipped Date

Apr 30, 2012  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jan 12, 2012