Clone

Target
IDP05083  
Clone Suffix
2
Experiment Start Date
Nov 16, 2011  
Experiment End Date
Nov 16, 2011  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
failed  
Vector
pMCSG7  
Sequence Start
35  
Sequence End
1310  
Reference Sequence Used
EETKKKETQK KANTETAKQL SKEEYAKLSA EEGLGNVLEA TVTDNVITLK IDNGDEAEDD YLEISPLANN LLKVNLRPRN IENSPSTPMI DPNATFTAIG ATIDLESDPM IISTDECTLE IDKEPARMTF KDKNGKTLLS ESADGGIFDG GVRFKRGASD HVYGAYGISF SDGDLGMERD DTAADSNKTA QAGQQGNAGG PFMWSTAGYG LLVDSDGGYP YSVSDEEKLE FYYGDTIVEG RRYVKENVEM YLMSGEPEEI MSSYADVTGH TPMMPKWSLG FSNFEWGTNE AEFRENINTY RAKGIPIDSF AFDYDWKFYG DPTYGGDYGE FAWNTENFPS AKDTTLKSDM DALGVKMIGI TKPRVVTNLE DGTATQQGKD ADANGYWYPG QSAYEDYAFP VTVRSIDPYN ADERNWWWSH SEDALDKGIV GWWNDETDKV SSNGADYWFG NFTTGFTSEA MYEGQRDYTN ERVWQTGRTF YPGAQRYSTS TWSGDIGVQW KKGEVIDWAN GMQEQPSTML STINLGQTKW GMDTGGFNAN SGQVLNPDPE LYSRWMEFSS LVPVFRTHGN QNQQRQPWFY GGTAEEVAKS SMTWRYSMIP YMYAYERQAY DSGVGLVQPL TFDNPTDENV VNLTDEWMFG DGLLAAPVLE EGAGSRDIYL PAGTWIDYNR GDVYEGNQTI NYEVNDDDWT DVPMFVKQGA IMPMQQPQNY VGESAVKTIY LDTFASDKET SFTHYDDDGK SFDYEKDTYL KQKMTTSKTA DTSTFTIGAK EGSYKADTEN YIVKLHGNAA ESVQVNGADS KVYDDLSALE NADGSGYAVG SDVYGAVTYV KVPAQADADT VITTSGSASI VQTGTYEMET GSNFADTVAD KPVAEKTYVD GYDKDGAGTT IYANVKDSGD YNVDLTYKNA SSDNQALSIF VNGEYVKQTT LKPNTDWSVQ SETLPLSAGK NSISYKVVTD TGDKADQVSL DKVNIGFTPT VAKVEAEEAD LAGTLKVAND HWFYSGEGFV GGFGTAGDEI KFEVDVPADG EYVLNTRTAN GTGMPQTLDL YTNGDYNSRV TFPSKGENWN IWSDTEKAVN LKAGTNKITF LRDGETTGNV NIDRILVSAE PINSPVQSEV NLLDNGGFER DAAATTNWVE SHPDGQDKAY GVDSGSGVNP PESPAFGKQS AYFYLPGAYK QTLAQQIAVP VNNANYDVEA WVKVSKDDAA KAQMTVNNYD SSGGKTIDLV ADDVWHYVTI KDVKVTTGNI NTSFQMDSPG GTSIHIDNVR VMPSAE  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGGAAGAGACGAAGAAAAAAGAGA  
PCR Backward Primer
TTATCCACTTCCAATGTTATTCTGCAGATGGCATAACT