Clone

Target

IDP05577  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Nov 16, 2011  

Experiment End Date

Nov 16, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

24  

Sequence End

172  

Reference Sequence Used

GKEQKTEKQT GNQSTSVEKI SDNILDEMEG PPKNGHTIER HVGKSEEDLK NRLKTDKVSA ASTYYDKETA TKAVKDSLKQ HDKEIQDWLK NSKEARLVLN TTHSFPVGKT VIKKNMNVKD KLVKTVTVLA RDKSGDLGYK IITSYPSDK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGGGAAAAGAACAAAAAACAGAGAA  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTATCAGATGGATAAGAAGT  

Shipped To Lab

UT  

Shipped Date

Jan 17, 2012  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jan 12, 2012