Clone

Target

IDP90914  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Nov 02, 2011  

Experiment End Date

Nov 02, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

opMCSG28  

Sequence Start

1  

Sequence End

545  

Reference Sequence Used

MKPTLLLHTS YDMQSSSEHI SDVLIIGSGA AGLSLALRLA PHCKVTVLSK GPLNEGATFY AQGGIAAVFD ETDSISSHVD DTLIAGAGLC DKEAVEFIAS NARSCVQWLI DQGVLFDTET SANGEERYHL TREGGHSHRR ILHTADATGK EVETTLVGKA SNHPNISVKE RCNAVDLITS NKIGLAGTKR VVGAYVWNRE LEKVETFRAK AVVLATGGAA KVYQYTTNPD ISSGDGIAMA WRAGCRVANL EFNQFHPTCL FHPQARNFLL TEALRGEGAY LKRPDGSRFM LDFDPRGELA PRDIVARAID HEMKRLGADC MYLDISHKPT DFVMQHFPMI YEKLLSLGID LTKEAIPIVP AAHYTCGGVM VDQHGRTDLD GLYAIGEVSY TGLHGANRMA SNSLLECLVY GWSAAEDILT RLPTAKLAKH LPDWDESRVD NSDERVVIQH NWHELRLFMW DYVGIVRTTK RLERALRRIN TLQQEIDEYY ANFRISNNLL ELRNLVQVAE LIVRCAMERK ESRGLHYTLD YPDQIENPQP TILHP  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCATGAAACCAACTCTTCTTCTGC  

PCR Backward Primer

GGTTCTCCCCACGTGGATGCAGAATGGTTGGCT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jan 12, 2012