Clone

Target

IDP00947  

Clone Suffix

2 - The clone is available in BEIR but it is out of stock.

Sequence Comments

211: Q -> R  

Experiment Start Date

Sep 04, 2008  

Experiment End Date

Sep 05, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

248  

Reference Sequence Used

MSFVVIIPAR FSSTRLPGKP LVDINGKPMI VHVLERARES GAERIIVATD HEDVARAVEA AGGEVCMTRA DHQSGTERLA EVVEKCGFSD DTVIVNVQGD EPMIPAVIIR QVAENLAQRQ VGMATLAVPI HSAEEAFNPN AVKVVLDAEG YALYFSRATI PWDRDRFAKS LETVGDTCLR HLGIYGYRAG FIRRYVSWQP SPLEHIEMLE QLRVLWYGEK IHVAVAKAVP GTGVDTADDL ERVRAEMR  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MSFVVIIPAR FSSTRLPGKP LVDINGKPMI VHVLERARES GAERIIVATD HEDVARAVEA AGGEVCMTRA DHQSGTERLA EVVEKCGFSD DTVIVNVQGD EPMIPAVIIR QVAENLAQRQ VGMATLAVPI HSAEEAFNPN AVKVVLDAEG YALYFSRATI PWDRDRFAKS LETVGDTCLR HLGIYGYRAG FIRRYVSWQP SPLEHIEMLE RLRVLWYGEK IHVAVAKAVP GTGVDTADDL ERVRAEMR  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGTTTCGTCGTCATCATTC  

PCR Backward Primer

TTATCCACTTCCAATGTTAACGCATTTCAGCGCGCA  

Shipped To Lab

UTSW  

Shipped Date

Oct 09, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008