Clone

Target
IDP00259  
Clone Suffix
2
Experiment Start Date
Sep 25, 2012  
Experiment End Date
Sep 28, 2012  
Person
Sofiya Shatsman  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
160  
Reference Sequence Used
MHLQYYAISL VSLVSLIDCH KLAFNFNLEI NGSDTHSTVD VYLDDSQIIT FDGKDIRPTI PFMIGDEIFL PFYKNVFSEF FSLFRRVPTS TPYEDLTYFY ECDYTDNKST FDQFYLYNGE EYTVKTQEAT NKNMWLTTSE FRLKKWFDGE DCIMHLRSLV  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGCATCTACAATATTATGCGAT  
PCR Backward Primer
TTATCCACTTCCAATGTTAAACTAACGATCTAAGATGCA