Clone

Target
IDP00888  
Clone Suffix
2
Experiment Start Date
Sep 04, 2008  
Experiment End Date
Sep 05, 2008  
Person
Jason Stam  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG19c  
Sequence Start
1  
Sequence End
76  
Reference Sequence Used
MKINNGPVLC PHCGCLSAYY EIDRLAAIRE KVNKEGGSAA WDSTLQAHKK KAFCLMCHKS IDEVVIGQSD APESTK  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAAAATAAATAATGGCCCCG  
PCR Backward Primer
TTATCCACTTCCAATGTTACTTCGTAGACTCAGGCG  
Shipped To Lab
UT  
Shipped Date
Oct 20, 2008  
Inserted
Oct 13, 2008  

Related Expressions

       
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2008-09-16 2008-09-17 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli M9 minimal 1mM IPTG assay
2008-10-14 2008-10-15 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2012-03-06 Maite Garcia University of Calgary Expression_protocol_Toronto success E. coli 15N 2X M9 none production
2010-10-29 Olena Onopriyenko University of Calgary Expression_protocol_Toronto success E. coli Se-Met none production