Clone

Target

IDP91695  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Aug 02, 2012  

Experiment End Date

Aug 02, 2012  

Person

Brian Bishop  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

2  

Sequence End

538  

Reference Sequence Used

TTPGKENFRL KSYKNKSLNP DEMRRRREEE GLQLRKQKRE EQLFKRRNVA TAEEETEEEV MSDGGFHEAQ ISNMEMAPGG VITSDMIEMI FSKSPEQQLS ATQKFRKLLS KEPNPPIDEV ISTPGVVARF VEFLKRKENC TLQFESAWVL TNIASGNSLQ TRIVIQAGAV PIFIELLSSE FEDVQEQAVW ALGNIAGDST MCRDYVLDCN ILPPLLQLFS KQNRLTMTRN AVWALSNLCR GKSPPPEFAK VSPCLNVLSW LLFVSDTDVL ADACWALSYL SDGPNDKIQA VIDAGVCRRL VELLMHNDYK VVSPALRAVG NIVTGDDIQT QVILNCSALQ SLLHLLSSPK ESIKKEACWT ISNITAGNRA QIQTVIDANI FPALISILQT AEFRTRKEAA WAITNATSGG SAEQIKYLVE LGCIKPLCDL LTVMDSKIVQ VALNGLENIL RLGEQEAKRN GTGINPYCAL IEEAYGLDKI EFLQSHENQE IYQKAFDLIE HYFGTEDEDS SIAPQVDLNQ QQYIFQQCEA PMEGFQL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGACCACCCCAGGAAAAGAGAA  

PCR Backward Primer

TTATCCACTTCCAATGTTAAAGCTGGAAACCTTCCATA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 29, 2012