Clone

Target
IDP91746  
Clone Suffix
1
Experiment Start Date
Aug 02, 2012  
Experiment End Date
Aug 02, 2012  
Person
Brian Bishop  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
2  
Sequence End
500  
Reference Sequence Used
ANIINLWNGI VPTVQDVNVA SITAFKSMID ETWDKKIEAN TCISRKHRNI IHEVIRDFMK AYPKMDENRK SPLGAPMQWL TQYYILKNEY YKTMLAYDNG SLNTKFKTLN IYMITNVGQY ILYIVFCIIS GKNHDGTPYI YDSEITSNDK NFINERIKYA CKQILHGQLT IALRIRNKFM FIGSPMYLWF NVNGSQVYHD IYDRNAGFHN KEIGRLLYAF MYYLSISGRF LNDFALLKFT YLGESWTFSL SVPEYILYGL GYSVFDTIEK FSNDAILVYI RTNNRNGYDY VEFNKKGIAK VTEDKPDNDK RIHAIRLIND STDVQHIHFG FRNMVIIDNE CANIQSSAEN ATDTGHHQDS KINIEVEDDV IDDDDYNPKP TPIPEPHPRP PFPRHEYHKR PKLLPVEEPD PVKKDADRIR LDNHILNTLD HNLNFIGHYC CDTAAVDRLE HHIETLGQYA VILARKINMQ TLLFPWPLPT VHPHAIDGSI PPHGRSTIL  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGGCGAACATTATAAATTTATGGAAC  
PCR Backward Primer
TTATCCACTTCCAATGTTATAAAATCGTAGATCTCCCAT  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2012-08-13 2012-08-13 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2012-08-20 2012-08-20 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay
2012-08-21 2012-08-21 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay