Clone

Target
IDP92619  
Clone Suffix
500
Experiment Start Date
Jan 03, 2012  
Experiment End Date
Jan 19, 2012  
Person
Jing Hou  
Lab
University of Virginia  
Protocol ID
uva_cloning_1  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
308  
Reference Sequence Used
MSWLEKILEK SNIVSSRKAS IPEGVWTKCT SCEQVLYYAE LERNLEVCPK CDHHMRMKAR RRLETFLDQG ERVELGEELE PQDKLKFKDS KRYKERLAAA QKASGEKDAL VVMKGAVLGV PVVACAFEFS FMGGSMGSVV GARFVRAVEA AMEHNCGLIC FSASGGARMQ EALMSLMQMA KTSAALERLS DKGLPFISVM TDPTMGGVSA SLAMLGDINI GEPKALIGFA GRRVIEQTVR EELPEGFQRS EFLLEHGAID MIVDRRDMRQ RVASLLAKMT GQASPLVVSV NDAPNEAPYA VPEANKKG  
PCR Forward Primer
 
PCR Backward Primer
 

Related Expressions

                         
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2012-07-03 2012-07-06 Jing Hou University of Virginia uva_expression_native_1 success E.coli LB IPTG production
2012-09-17 2012-09-19 Jing Hou University of Virginia uva_expression_semet_1 success E.coli LB-supplemented with 60mg ZnCl2 IPTG production
2012-09-17 2012-09-19 Jing Hou University of Virginia uva_expression_native_1 success E.coli LB-supplemented with 0.5mM Bitoin IPTG production
2012-09-24 2012-09-26 Jing Hou University of Virginia uva_expression_native_1 success E.coli LB-supplemented with biotin 222mg/L Bitoin IPTG production
2012-12-12 2012-12-14 Jing Hou University of Virginia uva_expression_native_1 success E.coli LB-supplemented with biotin 222mg/L Bitoin IPTG production
2013-01-30 2013-02-01 Jing Hou University of Virginia uva_expression_native_1 success E.coli LB-supplemented with biotin 222mg/L Bitoin IPTG production
2015-02-09 2015-02-11 Jing Hou University of Virginia allergens_purification_1 success E. coli LB IPTG production
2015-03-18 2015-03-20 Jing Hou University of Virginia uva_expression_native_1 success E. coli LB IPTG production
2015-03-29 2015-03-31 Jing Hou University of Virginia uva_expression_native_1 success E. coli LB IPTG production
2015-03-29 2015-03-31 Jing Hou University of Virginia uva_expression_native_1 success E. coli LB IPTG production
2015-04-27 2015-04-29 Jing Hou University of Virginia uva_expression_native_1 success E. coli LB IPTG production
2015-06-08 2015-06-10 Jing Hou University of Virginia uva_expression_native_1 success E. coli LB IPTG production
2016-03-07 2016-03-10 Jing Hou University of Virginia uva_expression_native_1 success E.coli LB IPTG production