Clone

Target

IDP01016  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Sep 04, 2008  

Experiment End Date

Sep 05, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

427  

Reference Sequence Used

MNTNNALMQR RHNAVPRGVG QIHPIFAERA ENCRVWDVEG REYLDFAGGI AVLNTGHLHP GIVSAVEAQL KKLSHTCFQV LAYEPYLALC ERMNQKVPGD FAKKTLLVTT GSEAVENAVK IARAATKRSG AIAFSGAYHG RTHYTLSLTG KVHPYSAGMG LMPGHVYRAL YPCPLHNISD DDAIASIERI FKNDAAPEDI AAIIIEPVQG EGGFYAASPA FMQRLRALCD QHGIMLIADE VQSGAGRTGT LFAMEQMGVA ADITTFAKSI AGGFPLAGVT GRADVMDAIA PGGLGGTYAG NPIACAAALA VLDIFEQENL LQKANTLGKT LRDGLMEIAE THREIGDVRG LGAMIAIELF ENGDPGKPNA ALTADIVTRA REKGLILLSC GPYYNILRIL VPLTIEASQI RQGLEIIAQC FDEAKQA  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAATACCAATAACGCTTTAATG  

PCR Backward Primer

TTATCCACTTCCAATGTTACGCTTGTTTCGCCTCATC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008