Clone

Target

IDP01080  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Sep 04, 2008  

Experiment End Date

Sep 05, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

449  

Reference Sequence Used

MTEIVVSKFG GTSVADFDAM NRSADIVLSD TNVRLVVLSA SAGITNLLVA LAEGMEPGER FATLDAIRKI QFDILDRLRH PNVIREEIER LLENITILAE AASLATSAAL TDELVSHGEL MSTLLFVEIL RERDVQAHWF DVRKVMRTSD RFGRAEPDVA ALAELAAQQL LPRLSETLVI TQGFIGSESK GRTTTLGRGG SDYTAALLAE ALHAARVDIW TDVPGIYTTD PRVVPVAQRI DEIDFEEAAE MATFGAKVLH PATLLPAVRS DIPVFVGSSK DPQAGGTLVC NKTQNPPLFR ALALRRNQTL LTLHSLNMLH SRGFLAEVFG ILARHNISVD LITTSEVSVA LTLDTTGSTS TGDTLLTQSL LMELSALCRV EVEEGLALVA LIGNNLSKAC GVGKEVFGVL EPFNIRMICY GASSHNLCFL VPGDDAEKVV QKLHQNLFE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGACAGAGATCGTTGTTTCC  

PCR Backward Primer

TTATCCACTTCCAATGTTATTCAAATAAGTTCTGATGTAG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008