Clone

Target
IDP01024  
Clone Suffix
1
Experiment Start Date
Sep 08, 2008  
Experiment End Date
Sep 09, 2008  
Person
Jason Stam  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
97  
Reference Sequence Used
MSIDRTSPLK PVSTVQTRET SDTPVQKTRQ EKTSAATSAS VTLSDAQAKL MQPGVSDINM ERVEALKTAI RNGELKMDTG KIADSLIREA QSYLQSK  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAGCATTGACCGTACCTC  
PCR Backward Primer
TTATCCACTTCCAATGTTATTTACTCTGTAAGTAGCTCT  
Shipped To Lab
ANL  
Shipped Date
Oct 09, 2008  
Inserted
Oct 13, 2008  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2008-09-15 2008-09-16 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli M9 minimal 1mM IPTG assay
2008-10-13 2008-10-14 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay
2008-11-05 2008-11-08 Min Zhou Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal + semet 1 mM IPTG production