Clone

Target
IDP06331  
Clone Suffix
104
Sequence Comments
short CBS deletion  
Experiment Start Date
Nov 01, 2012  
Experiment End Date
Nov 02, 2012  
Person
Jerzy Osipiuk  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG50  
Sequence Start
1  
Sequence End
394  
Reference Sequence Used
MAFYFEEPSR TFSEFLLVPG YSSAECVPTN VSLKTPIVKF KKGEESAITM NIPLVSAIMQ AVSDDNMGIA LATEGGVSFI FGSQSIESEA AMVSRVKNHK SGGRKDYDSN KENKLELLDS SKRYVVGAGI NTRDYEERVP ALVEAGADIL CIDSSEGYSE WQKRTLDYVR GKYGDTVKVG AGNVVDRDGF RYLAEAGADF VKVGVGGGSI CITREQKGIG RGQATALIDV AKARDEYFEE TGVYIPICSD GGIVYDYHMT LALAMGADFI MLGRYFSRFD ESPTNKVNLN GTYMKEYWGE GANRARNWQR YDLGGDKKLS FEEGVDSYVP YAGSLKDNVA ISLSKVRSTM CNCGALNIPE LQQKAKITLV SSTSIVEGGA HDVVVKDASN NLIK  
PCR Forward Primer
TACTTCCAATCCAATGCCATGGCATTTTATTTTGAAGAACCGTCCC  
PCR Backward Primer
TTATCCACTTCCAATGTTATTTGATTAAATTGTTCGAAGCATCTTTCACAACAA  
Shipped To Lab
ANL  
Shipped Date
Nov 02, 2012  
Inserted
Aug 08, 2013  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2013-10-28 2013-10-30 Magdalena Makowska-Grzyska Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 0.5 mM IPTG production
2013-10-29 2013-10-31 Magdalena Makowska-Grzyska Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 0.5 mM IPTG production
2012-11-04 2012-11-06 Jerzy Osipiuk Argonne National Laboratory standard expression_ANL success E. coli M9 minimal 1 mM IPTG assay