Clone

Target

IDP91076  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Sep 24, 2013  

Experiment End Date

Sep 24, 2013  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

587  

Reference Sequence Used

MQEQKLQEND FSTLQTFKRL WPMIKPFKAG LIVSGVALVF NALADSGLIY LLKPLLDDGF GKANHSFLKM MAFVVVGMII LRGITNFISN YCLAWVSGKV VMTMRRRLFK HLMFMPVSFF DQNSTGRLLS RITYDSEEIA SSSSGSLITI VREGAYIISL FAVMFYTSWE LTIVLFIIGP IIAVLIRLVS KIFRRLSKNL QDSMGELTSA TEQMLKGHKV VLSFGGQHVE EVHFNHVSND MRRKSMKMVT ANSISDPVVQ VIASLALATV LYLATTPLIA EDNLSAGSFT VVFSSMLAMM RPLKSLTAVN AQFQSGMAAC QTLFAILDLE PEKDDGAYKA EPAKGELEFK NVSFAYQGKE ELALNNISFS VPAGKTVALV GRSGSGKSTI ANLVTRFYDI EQGEILLDGV NIQDYRLSNL RENCAVVSQQ VHLFNDTIAN NIAYAAQDKY SREEIIAAAK AAYALEFIEK LPQGFDTVIG ENGASLSGGQ RQRLAIARAL LRNSPVLILD EATSALDTES ERAIQSALEE LKKDRTVVVI AHRLSTIENA DEILVIDHGE IRERGNHKTL LEQNGAYKQL HSMQFTG  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCAAGAGCAAAAATTACAAGA  

PCR Backward Primer

TTATCCACTTCCAATGTTAGCCAGTAAACTGCATACTA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2013