Clone

Target

IDP91083  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Sep 24, 2013  

Experiment End Date

Sep 24, 2013  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

586  

Reference Sequence Used

MQGIKRYLQF VKPYRWLIAI TIMIGLVKFG IPLIMPWLLK YIIDDVIQGA GSLQDKTSQL VTAIGIAFFI FAVLRPPIEY YRQYFAQRIA NTILYDLRKH IFGHLQKLSL RYYSNTKTGE IISRVIHDVE QTKDFVITGL MNVWLDTATI AIAIIVMFSM NIKLTFVALL ILPIYVVAVK YFFGRLRKLT KERSQALATM QGYLHERIQG MQVTRSFALE EYEGKQFEKR NNEFLTKALT HTSWTARTFS AVNTLTDLGP LLVIGFAAYE VIQGQLTLGT MVAFVGYMDS LYSPLRRLVN SSTTLTQSFA SMDRVFELLD EKYDIVNVPS AVQTKKLNGE VIFDNVSFRY NADEKEILHN LSLTMLPGEK VALVGASGGG KSSLASLIPR FYDVSSGAVY VDGIDVRKYD MRNLRSHIGI VLQDNLLFSD TIGANILYGN PKATEEEVIA AAKAAQIHDF IIELPDGYDT VVGERGVKLS GGQRQRVAIA RVFLKNPSLL ILDEATPALD LENERYIQEA LQTLAADRTT IIIAHRLATI THVDTIIYIE DGEIKETGSH EELMKKRGYY YNLYQLQHIT ETAPLA  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCAAGGTATAAAAAGATATTTG  

PCR Backward Primer

TTATCCACTTCCAATGTTACGCTAAAGGAGCTGTTTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2013