Clone

Target

IDP00418  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

437  

Reference Sequence Used

MTQQEYQNRR QALLAKMAPG SAAIIFAAPE ATRSADSEYP YRQNSDFSYL TGFNEPEAVL ILVKSDETHN HSVLFNRIRD LTAEIWFGRR LGQEAAPTKL AVDRALPFDE INEQLYLLLN RLDVIYHAQG QYAYADNIVF AALEKLRHGF RKNLRAPATL TDWRPWLHEM RLFKSAEEIA VLRRAGEISA LAHTRAMEKC RPGMFEYQLE GEILHEFTRH GARYPAYNTI VGGGENGCIL HYTENECELR DGDLVLIDAG CEYRGYAGDI TRTFPVNGKF TPAQRAVYDI VLAAINKSLT LFRPGTSIRE VTEEVVRIMV VGLVELGILK GDIEQLIAEQ AHRPFFMHGL SHWLGMDVHD VGDYGSSDRG RILEPGMVLT VEPGLYIAPD ADVPPQYRGI GIRIEDDIVI TATGNENLTA SVVKDPDDIE ALMALNH  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MTQQEYQNRR QALLAKMAPG SAAIIFAAPE ATRSADSEYP YRQNSDFSYL TGFNEPEAVL ILVKSDETHN HSVLFNRIRD LTAEIWFGRR LGQEAAPTKL AVDRALPFDE INEQLYLLLN RLDVIYHAQG QYAYADNIVF AALEKLRHGF RKNLRAPATL TDWRPWLHEM RLFKSAEEIA VLRRAGEISA LAHTRAMEKC RPGMFEYQLE GEILHEFTRH GARYPAYNTI VGGGENGCIL HYTENECELR DGDLVLIDAG CEYRGYAGDI TRTFPVNGKF TPAQRAVYDI VLAAINKSLT LFRPGTSIRE VTEEVVRIMV VGLVELGILK GDIEQLIAEQ AHRPFFMHGL SHWLGMDVHD VGDYGSSDRG RILEPGMVLT VEPGLYIAPD ADVPPQYRGI GIRIEDDIVI TATGNENLTA SVVKDPDDIE ALMA*IT  

PCR Forward Primer

TACTTCCAATCCAATGCGATGACTCAGCAAGAATACCAG  

PCR Backward Primer

TTATCCACTTCCAATGTCAGTGATTCAATGCCATCAAT  

Shipped To Lab

ANL  

Shipped Date

Jul 16, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008