Clone

Target

IDP00269  

Clone Suffix

301

Sequence Comments

Cloned NheI/NotI into pet21a(+)  

Experiment Start Date

Jan 20, 2011  

Experiment End Date

Jan 27, 2011  

Person

Chris Nelson  

Lab

Washington University  

Protocol ID

WU_basic_cloning_protocol_1  

Status

success  

Vector

pET21A(+)  

Sequence Start

172  

Sequence End

352  

Reference Sequence Used

MRDSYTQVCD HRLMSAAIKD SKAVHADMGY WIESEKNETW KLARASFIEV KTCIWPKSHT LWSNGVLESE MIIPKIYGGP ISQHNYRPGY FTQTAGPWHL GKLELDFDLC EGTTVVVDEH CGNRGPSLRT TTVTGKTIHE WCCRSCTLPP LRFKGEDGCW YGMEIRPVKE KEENLVKSMV SA  

Reference Sequence Variance

 

Confirmed Protein Sequence

 

PCR Forward Primer

 

PCR Backward Primer

 

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jan 23, 2014