Clone

Target

IDP92741  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Mar 10, 2014  

Experiment End Date

Mar 11, 2014  

Person

Wayne Anderson  

Lab

Northwestern University  

Protocol ID

NU_default_cloning  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

424  

Reference Sequence Used

MAKDKGFFKK AKTGKNKSSA TPYTEECAIQ IPSEIDNEQM HRMPAGGEED QYSRIKHMSA LIKKYGNLPI ITTQETSLPD YWLDLFAAID EGDIPKAHTL FHLLPQDDII LRALRAVHSE DYLYQLIKYC IQAKHFGFKQ LNADLVVTPK TFEILIKDCA ATLFNPAKTH FSFGLPSHHA YTQMGSGFCL INKTAMLIKQ AELSSVQPPR FVIIGTDVNR DNGLCDILRH SFSHLSICHI DVFDSRVYPQ QDFSYINNEF NSEGVDVGKN IHVWHHNDLS YYAVDLSLTS RKSVGVHPAL LFALEQFKAS IREAKVKGQK IALYLPTGWD SHEDETAYCG KFVKGRMLGK TAAHQFRFND GDLSYFYESI FTLYNENKDC IDTIYWGLEG GYDRTMYERE LTLLLQVIEK QLLPQDSNSH NMSC  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MAKDKGFFKK AKTGKNKSSA TPYTEECAIQ IPSEIDNEQM HRMPAGGEED QYSRIKHMSA LIKKYGNLPI ITTQETSLPD YWLDLFAAID EGDIPKAHTL FHLLPQDDII LRALRAVHSE DYLYQLIKYC IQAKHFGFKQ LNADLVVTPK TFEILIKDCA ATLFNPAKTH FSFGLPSHHA YTQMGSGFCL INKTAMLIKQ AELSSVQPPR FVIIGTDVNR DNGLCDILRH SFSHLSICHI DVFDSRVYPQ QDFSYINNEF NSEGVDVGKN IHVWHHNDLS YYAVDLSLTS RKSVGVHPAL LFALEQFKAS IREAKVKGQK IALYLPTGWD SHEDETAYCG KFVKGRMLGK TAAHQFRFND GDLSYFYESI FTLYNENKDC IDTIYWGLEG GYDRTMYERE LTLLLQVIEK QLLPQDSNSH NMSC  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGCCAAGGATAAAGGATTTT    

PCR Backward Primer

TTATCCACTTCCAATGTTAACAGGACATATTATGCGAAT  

Shipped To Lab

NU  

Shipped Date

Mar 11, 2014  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Mar 14, 2014