Clone

Target
IDP04427  
Clone Suffix
2
Experiment Start Date
Apr 24, 2014  
Experiment End Date
Apr 24, 2014  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG73  
Sequence Start
1  
Sequence End
256  
Reference Sequence Used
MEIKNVTFSY DNVTDRLKSV SSEIEIGKVT TIIGPNGCGK STLLGVMSRN HDPRSGEVIL DGKAISQYKP KEFARKLAVV HQQNEAPADM TVEKLTSFGR MPHKNIFSSQ TDEDREAIER ALACTNLLSK RDKEIHALSG GERQRVWIAM TLAQKTPMLF LDEPTTYLDI YYQLEILELV KELNEVHGLT IVMVLHDINQ AIRYSDHIIV MKDGEIVTKG KPDDVVTENM IKAIYGVDVV VKQDEDTGLY MVPMGI  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGGAAATTAAAAATGTAACCTTC  
PCR Backward Primer
TTATCCACTTCCAATGTTAAATACCCATTGGGACCATA  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2011-04-18 2011-04-20 Karolina Majorek University of Virginia uva_expression_semet_1 success E.coli SeMet IPTG production
2014-02-21 2014-02-22 Karol Szlachta University of Virginia uva_expression_native_1 success Ecoli LB 0.25 mM IPTG production