Clone

Target
IDP00791  
Clone Suffix
1
Experiment Start Date
May 01, 2014  
Experiment End Date
Jun 03, 2014  
Person
Salvatore Nocadello  
Lab
Northwestern University  
Protocol ID
Autoinduction HTMC1-HTMC2  
Status
success  
Vector
pMCSG53  
Sequence Start
40  
Sequence End
238  
Reference Sequence Used
SIGQDETNIN ELIKYYTQPH FSFSNKWLYQ YDNGNIYVEL KRYSWSAHIS LWGAESWGNI NQLKDRYVDV FGLKDKDTDQ LWWSYRETFT GGVTPAAKPS DKTYNLFVQY KDKLQTIIGA HKIYQGNKPV LTLKEIDFRA REALIKNKIL YNENRNKGKL KITGGGNNYT IDLSKRLHSD LANVYVKNPN KITVDVLFD  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
SIGQDETNIN ELIKYYTQPH FSFSNKWLYQ YDNGNIYVEL KRYSWSAHIS LWGAESWGNI NQLKDRYVDV FGLKDKDTDQ LWWSYRETFT GGVTPAAKPS DKTYNLFVQY KDKLQTIIGA HKIYQGNKPV LTLKEIDFRA REALIKNKIL YNENRNKGKL KITGGGNNYT IDLSKRLHSD LANVYVKNPN KITVDVLFD  
PCR Forward Primer
TACTTCCAATCCAATGCGAGCATTGGTCAAGATGAAACTA  
PCR Backward Primer
TTATCCACTTCCAATGCTAATCAAAGAGGACGTCAAC  
Shipped To Lab
NU  
Shipped Date
Jul 31, 2014  
Inserted
Jul 04, 2014  

Related Expressions

         
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2014-06-03 2014-06-03 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2014-08-25 2014-08-27 Salvatore Nocadello Northwestern University Autoinduction HTMC1-HTMC2 success E. coli TB IPTG production
2018-11-05 2018-11-07 Grant Wiersum Northwestern University Autoinduction HTMC1-HTMC2 success E.Coli TB IPTG production
2018-11-12 2018-11-14 Grant Wiersum Northwestern University NU_native_expression success E.Coli TB IPTG production
2019-02-04 2019-02-07 Grant Wiersum Northwestern University NU_SelMet_expression success E. Coli Selenomethionine IPTG production