Clone

Target

IDP05083  

Clone Suffix

6

Sequence Comments

 

Experiment Start Date

Aug 05, 2014  

Experiment End Date

Aug 05, 2014  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7-HaloV2  

Sequence Start

35  

Sequence End

1310  

Reference Sequence Used

EETKKKETQK KANTETAKQL SKEEYAKLSA EEGLGNVLEA TVTDNVITLK IDNGDEAEDD YLEISPLANN LLKVNLRPRN IENSPSTPMI DPNATFTAIG ATIDLESDPM IISTDECTLE IDKEPARMTF KDKNGKTLLS ESADGGIFDG GVRFKRGASD HVYGAYGISF SDGDLGMERD DTAADSNKTA QAGQQGNAGG PFMWSTAGYG LLVDSDGGYP YSVSDEEKLE FYYGDTIVEG RRYVKENVEM YLMSGEPEEI MSSYADVTGH TPMMPKWSLG FSNFEWGTNE AEFRENINTY RAKGIPIDSF AFDYDWKFYG DPTYGGDYGE FAWNTENFPS AKDTTLKSDM DALGVKMIGI TKPRVVTNLE DGTATQQGKD ADANGYWYPG QSAYEDYAFP VTVRSIDPYN ADERNWWWSH SEDALDKGIV GWWNDETDKV SSNGADYWFG NFTTGFTSEA MYEGQRDYTN ERVWQTGRTF YPGAQRYSTS TWSGDIGVQW KKGEVIDWAN GMQEQPSTML STINLGQTKW GMDTGGFNAN SGQVLNPDPE LYSRWMEFSS LVPVFRTHGN QNQQRQPWFY GGTAEEVAKS SMTWRYSMIP YMYAYERQAY DSGVGLVQPL TFDNPTDENV VNLTDEWMFG DGLLAAPVLE EGAGSRDIYL PAGTWIDYNR GDVYEGNQTI NYEVNDDDWT DVPMFVKQGA IMPMQQPQNY VGESAVKTIY LDTFASDKET SFTHYDDDGK SFDYEKDTYL KQKMTTSKTA DTSTFTIGAK EGSYKADTEN YIVKLHGNAA ESVQVNGADS KVYDDLSALE NADGSGYAVG SDVYGAVTYV KVPAQADADT VITTSGSASI VQTGTYEMET GSNFADTVAD KPVAEKTYVD GYDKDGAGTT IYANVKDSGD YNVDLTYKNA SSDNQALSIF VNGEYVKQTT LKPNTDWSVQ SETLPLSAGK NSISYKVVTD TGDKADQVSL DKVNIGFTPT VAKVEAEEAD LAGTLKVAND HWFYSGEGFV GGFGTAGDEI KFEVDVPADG EYVLNTRTAN GTGMPQTLDL YTNGDYNSRV TFPSKGENWN IWSDTEKAVN LKAGTNKITF LRDGETTGNV NIDRILVSAE PINSPVQSEV NLLDNGGFER DAAATTNWVE SHPDGQDKAY GVDSGSGVNP PESPAFGKQS AYFYLPGAYK QTLAQQIAVP VNNANYDVEA WVKVSKDDAA KAQMTVNNYD SSGGKTIDLV ADDVWHYVTI KDVKVTTGNI NTSFQMDSPG GTSIHIDNVR VMPSAE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGGAAGAGACGAAGAAAAAAGAGA  

PCR Backward Primer

TTATCCACTTCCAATGTTATTCTGCAGATGGCATAACT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 30, 2014