Clone

Target
IDP06499  
Clone Suffix
1
Experiment Start Date
Sep 30, 2014  
Experiment End Date
Oct 01, 2014  
Person
Wayne Anderson  
Lab
Northwestern University  
Protocol ID
NU_default_cloning  
Status
success  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
126  
Reference Sequence Used
MLEQARLKVE QQAIKNIQFL EQDLPKNPLE KEFDCLAVSR VLHHMPDLDA ALSLFHQHLK EDGKLIIADF TKTEANHHGF DLAELENKLI EHGFSSVHSQ ILYSAEDLFQ GNHSEFFLIV AQKSLA  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MLEQARLKVE QQAIKNIQFL EQDLPKNPLE KEFDCLAVSR VLHHMPDLDA ALSLFHQHLK EDGKLIIADF TKTEANHHGF DLAELENKLI EHGFSSVHSQ ILYSAEDLFQ GNHSEFFLIV AQKSLA  
PCR Forward Primer
 
PCR Backward Primer
 
Shipped To Lab
NU  
Shipped Date
Oct 01, 2014  
Inserted
Aug 30, 2014  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2014-08-05 2014-08-05 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2014-10-27 2014-10-28 Kristin Flores Northwestern University NU_SelMet_expression success E. coli SM IPTG production