Clone

Target
IDP95074  
Clone Suffix
1
Experiment Start Date
Sep 30, 2014  
Experiment End Date
Oct 01, 2014  
Person
Wayne Anderson  
Lab
Northwestern University  
Protocol ID
NU_default_cloning  
Status
success  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
136  
Reference Sequence Used
MKIKKSLISA MMVSAMVLGG TGAVFANGND SSNIALPKFE SLIKSGITDP TELEKYGVEH YTYEEYAKHI QELKDYAKDP NAVKDVSQKD LEETIKKMEQ ELEKIKTEGL KIMKPITIEN EDGSQISIAY NYAAID  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
DSSNIALPKF ESLIKSGITD PTELEKYGVE HYTYEEYAKH IQELKDYAKD PNAVKDVSQK DLEETIKKME QELEKIKTEG LKIMKPITIE NEDGSQISIA YNYAAID  
PCR Forward Primer
 
PCR Backward Primer
 
Shipped To Lab
NU  
Shipped Date
Oct 01, 2014  
Secondary Shipped To Lab
NU  
Secondary Shipped Date
Mar 30, 2016  
Inserted
Aug 30, 2014  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2014-08-05 2014-08-05 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2014-10-27 2014-10-28 Kristin Flores Northwestern University NU_SelMet_expression success E. coli SM IPTG production
2016-04-26 2016-04-28 Olga Kiryukhina Northwestern University NU_SelMet_expression success E. coli SeMet IPTG production