Clone

Target
IDP95282  
Clone Suffix
1
Experiment Start Date
Jan 06, 2015  
Experiment End Date
Jan 07, 2015  
Person
Wayne Anderson  
Lab
Northwestern University  
Protocol ID
NU_default_cloning  
Status
success  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
165  
Reference Sequence Used
MHLFELQDAY QQILRDTLQI IPLSANQGGQ LHPALEIDNQ ILRFSKAGVT DPLKQGLSPF ERIEYRYDAD QKKLYRLKYT NLNTSNREQP LSSTLLNQVD QYQIMVLTPQ EVTKWPEVNI DPTKPNELKK LPKGIKIQLT VAGVNYEWIY SLNQGDLSLS QEGNS  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MHLFELQDAY QQILRDTLQI IPLSANQGGQ LHPALEIDNQ ILRFSKAGVT DPLKQGLSPF ERIEYRYDAD QKKLYRLKYT NLNTSNREQP LSSTLLNQVD QYQIMVLTPQ EVTKWPEVNI DPTKPNELKK LPKGIKIQLT VAGVNYEWIY SLNQGDLSLS QEGNS  
PCR Forward Primer
 
PCR Backward Primer
 
Shipped To Lab
NU  
Shipped Date
Jan 07, 2015  
Inserted
Oct 09, 2014  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2014-09-26 2014-09-26 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2015-01-27 2015-01-28 Kristin Flores Northwestern University NU_SelMet_expression success E. coli SM IPTG production