Clone

Target
IDP06557  
Clone Suffix
107
Sequence Comments
IDP06557.22-259.P(261)..pMCSG68  
Experiment Start Date
May 15, 2015  
Experiment End Date
May 26, 2015  
Person
Michael Endres  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG68  
Sequence Start
22  
Sequence End
259  
Reference Sequence Used
IRSSVAEQEA DRDIIRFGEV SFSQLAEGVW MHTTYLDLMG FGPIPSNGLL VVNGDNTILV DTAWTDEQTE QIVAWASMVL AKPVRAAVVT HAHQDKMGGM AALHGANIAT WAHPLSNELA PEEGLVPARN AITFDANGWA TGEAAQSLAP LRLYYPGGAH TRDNITVGLP ELGIAFGGCM IKAGDASNLG NLADADTAAY AQSVRNFAAA FPDARTIAMS HSPPEGRKAI ERTLDLAE  
PCR Forward Primer
TACTTCCAATCCAATGCCATTCGCTCCTCCGTGGCTGAA  
PCR Backward Primer
TTATCCACTTCCAATGTTATTCAGCCAGGTCCAGCGTGC  
Shipped To Lab
ANL  
Shipped Date
May 26, 2015  
Inserted
Nov 04, 2014  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2014-09-12 2014-09-12 Shonda Clancy Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay
2015-05-27 2015-05-27 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay
2015-06-15 2015-06-17 Rory Mulligan Argonne National Laboratory standard expression_ANL success E. coli M9 minimal 1 mM IPTG production