Clone

Target
IDP06558  
Clone Suffix
105
Sequence Comments
IDP06558.30-261.P(262)..pMCSG68  
Experiment Start Date
May 15, 2015  
Experiment End Date
May 26, 2015  
Person
Michael Endres  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG68  
Sequence Start
30  
Sequence End
261  
Reference Sequence Used
QSEQSTHIEG VAEKPVEFVK LGTGVWMHTG YKVVPPWGNI RTNGLIIERG DYSVLVDTAW NDAQTAEIVA WAKDTLQKPI RASIHTHAHS DKMGGMDALH MLGVETFATD LTNRLAIERG LMPAKNVLNI SEIGSQIEWE GLTILYPGGG HSEDNIVVNE GVNNILFGGC MIRPGMTTSL GNIDDANLGY WSKAVENAAN AFPDSQIVIP SHGKPAGREI LKNTAYIARP KL  
PCR Forward Primer
TACTTCCAATCCAATGCCCAGAGCGAACAATCTACGCACATTGAA  
PCR Backward Primer
TTATCCACTTCCAATGTTACAGTTTCGGGCGAGCGATGTAT  
Shipped To Lab
ANL  
Shipped Date
May 26, 2015  
Inserted
Nov 04, 2014  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2014-09-12 2014-09-12 Shonda Clancy Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay
2015-05-27 2015-05-27 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay
2015-06-15 2015-06-17 Rory Mulligan Argonne National Laboratory standard expression_ANL success E. coli M9 minimal 1 mM IPTG production