Clone

Target
IDP06558  
Clone Suffix
139
Sequence Comments
IDP06558.32-257.P(262)..pMCSG73  
Experiment Start Date
May 15, 2015  
Experiment End Date
May 26, 2015  
Person
Michael Endres  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
failed  
Vector
pMCSG73  
Sequence Start
32  
Sequence End
257  
Reference Sequence Used
EQSTHIEGVA EKPVEFVKLG TGVWMHTGYK VVPPWGNIRT NGLIIERGDY SVLVDTAWND AQTAEIVAWA KDTLQKPIRA SIHTHAHSDK MGGMDALHML GVETFATDLT NRLAIERGLM PAKNVLNISE IGSQIEWEGL TILYPGGGHS EDNIVVNEGV NNILFGGCMI RPGMTTSLGN IDDANLGYWS KAVENAANAF PDSQIVIPSH GKPAGREILK NTAYIA  
PCR Forward Primer
TACTTCCAATCCAATGCCGAACAATCTACGCACATTGAAGGCGTT  
PCR Backward Primer
TTATCCACTTCCAATGTTAAGCGATGTATGCGGTGTTTTTCAGAATTT  
Shipped To Lab
ANL  
Shipped Date
May 26, 2015  
Inserted
Nov 04, 2014  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2014-09-12 2014-09-12 Shonda Clancy Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay
2015-05-27 2015-05-27 Michael Endres Argonne National Laboratory standard expression_ANL failed E. coli LB + 40 mM K2HPO4 1 mM IPTG assay