Clone

Target
IDP90140  
Clone Suffix
1
Sequence Comments
Removed signal peptide from N-terminus (MRSLIIVLLFPSIIYS) and the endoplasmic reticulum retention signal (KTEL) from the C-terminus. Added 6HIS tag at C-terminus.  
Experiment Start Date
Apr 20, 2009  
Experiment End Date
Apr 20, 2009  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
221  
Reference Sequence Used
MRSLIIVLLF PSIIYSMSIR RCEKTEEETW GLKIGLCIIA KDFYPERTDC SVHRPTASGG LITEGNGFRV VIYDQCTEPH DFIITDTQQT RLGSSHTYIK FSNMNTGVPS SIPKCSRTLS ISVYCDQEAG DIKFEEYTQE SSDISIRVKY DSSCIDYLGI NQSFMNECIR RITTWDRESC VRIDTQTINK YLKSCTNTKF DRNVYKRYIL KSKALHAKTE L  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAGGTCCCTGATTATAGTC  
PCR Backward Primer
TTATCCACTTCCAATGTTACAACTCTGTTTTAGCATGG  
Shipped To Lab
WU  
Shipped Date
Feb 02, 2009  
Inserted
Feb 23, 2009