Clone

Target
IDP52513  
Clone Suffix
102
Sequence Comments
IDP52513.1-443.F..pMCSG73  
Experiment Start Date
May 12, 2016  
Experiment End Date
May 12, 2016  
Person
Gyorgy Babnigg  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG73  
Sequence Start
1  
Sequence End
443  
Reference Sequence Used
MNSIFSIIEH QAFSKEDLKE IFKEKAEGFY KELEDFAKNN ENLLGFKNKN SLKAKNYVGI IQTKSGVLEI LPKCTNLDSY KKEDSSSNHD KEKLRRCYEL DNISKNNDFY EKDFKFNPKN LLINMLKTLK NSPFKKSQIS SLQSSKIPLF EVFITMFLDE FDSVYKKGLM RSYLSCEENR AFLKGKLLFN EHIKQNLIHK ERFFTSNDEF VLDIAPNRLI KSTLNFLKSK TSLNKFRLIK AMQMLDEVEF SKNYEKDFSY KISRHFDYYE NLLLWCKIFL KNESFMPYHG KNEAFALLFP MEKIFEDYVA YMLKKVNSTQ DIKVQNNGKY LISKNDENCF MLKPDLYIKN KMILDTKWKI PNDSEDEKKQ GIAQSDLYQM FAYACKFKIY DIKLVYPLCE KTQDLQRKIA EKFFVFKASE HLYFKEQGQK DIKVQVFFAP LPF  
PCR Forward Primer
TACTTCCAATCCAATGCCATGAATAGTATTTTTTCCATCATCGAACACCAAG  
PCR Backward Primer
TTATCCACTTCCAATGTTAAAAAGGTAAAGGTGCAAAAAATACTTGGACTTTTATAT  
Shipped To Lab
ANL  
Shipped Date
May 27, 2016  
Inserted
May 31, 2016  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2016-05-27 2016-05-27 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay
2016-05-26 2016-05-28 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 1 mM IPTG production