Clone

Target
IDP55101  
Clone Suffix
121
Sequence Comments
IDP55101.37-270.P(270).Q37N,Q38Y,C208A.pMCSG7  
Experiment Start Date
May 12, 2016  
Experiment End Date
May 12, 2016  
Person
Gyorgy Babnigg  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG7  
Sequence Start
37  
Sequence End
270  
Reference Sequence Used
QQMETGDQRF GDLVFRQLAP NVWQHTSYLD MPGFGAVASN GLIVRDGGRV LVVDTAWTDD QTAQILNWIK QEINLPVALA VVTHAHQDKM GGMDALHAAG IATYANALSN QLAPQEGMVA AQHSLTFAAN GWVEPATAPN FGPLKVFYPG PGHTSDNITV GIDGTDIAFG GCLIKDSKAK SLGNLGDADT EHYAASARAF GAAFPKASMI VMSHSAPDSR AAITHTARMA DKLR  
PCR Forward Primer
TACTTCCAATCCAATGCCCAGCAAATGGAAACGGGCGATCA  
PCR Backward Primer
TTATCCACTTCCAATGTTAGCGCAGTTTGTCGGCCATACG  
Shipped To Lab
ANL  
Shipped Date
May 27, 2016  
Inserted
May 31, 2016  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2016-05-27 2016-05-27 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay
2016-05-25 2016-05-27 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 1 mM IPTG production