Clone

Target
IDP65002  
Clone Suffix
103
Sequence Comments
IDP65002.27-380.P(380)..pMCSG68  
Experiment Start Date
May 12, 2016  
Experiment End Date
May 12, 2016  
Person
Gyorgy Babnigg  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG68  
Sequence Start
27  
Sequence End
380  
Reference Sequence Used
DPSAAVARAF APLLDQYDVP GMAVAVTVDG RQHFYEFGVV SKQTQAPVTR DTLFEIGSVS KTFTATLAGY AATRGVLNLD DHPGRYLPAL AGTPIDRAEL RNLGTYTAGG LPLQFPESVT DDEQMIAYFQ QFQPVTAPGK IRQYSNPSVG LLGHISARAL GGQFTDLMQS QILTGLGLRR SFVDVTDEAM DFYAWGYDKK NHPVRVNPGV FDAEAYGVKS TTADMIRFIE HNIDPGALEP TLREAVKSTQ VGYYKVGPMV QDLGWEQYPY PVALDQLLAG NSGEMAMSPQ AATAIAPPSV GSALFNKTGS TDGFGAYAAF VPERRIGIVM LANKNFPIPA RVTAAHTVLD ALDA  
PCR Forward Primer
TACTTCCAATCCAATGCCGATCCCTCCGCAGCAGTTGC  
PCR Backward Primer
TTATCCACTTCCAATGTTAGGCGTCCAGAGCGTCAAGCA  
Shipped To Lab
ANL  
Shipped Date
May 27, 2016  
Inserted
May 31, 2016  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2016-05-27 2016-05-27 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay
2014-12-08 2014-12-10 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 1 mM IPTG production