Clone

Target
IDP65017  
Clone Suffix
102
Sequence Comments
IDP65017.30-294.P(294)..pMCSG73  
Experiment Start Date
May 12, 2016  
Experiment End Date
May 12, 2016  
Person
Gyorgy Babnigg  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG73  
Sequence Start
30  
Sequence End
294  
Reference Sequence Used
AIPGSLDKQL AALEHSANGR LGIAMINSGA GTKILYRGAQ RFPFCSTFKF MLAAAVLDQS QSQPNLLNKH INYHESDLLS YAPITRKNLA CGMTVSELCA ATIQYSDNTA ANLLIKELGG LAAVNQFARS IGDQMFRLDR WEPDLNTALP NDPRDTTTPA AMAASMNKLV LGDALRPAQR SQLAAWLKGN TTGDATIRAG APTDWIVGDK TGSGDYGTTN DIAVLWPTKG APIVLVVYFT QREKDAKPRR DVLASATQII LSQIS  
PCR Forward Primer
TACTTCCAATCCAATGCCGCGATTCCGGGATCATTAGACAAG  
PCR Backward Primer
TTATCCACTTCCAATGTTAGGATATTTGCGACAAAATTATTTGGGTCG  
Shipped To Lab
ANL  
Shipped Date
May 27, 2016  
Inserted
May 31, 2016  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2016-05-27 2016-05-27 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay
2016-05-21 2016-05-23 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 1 mM IPTG production