Clone

Target
IDP63266  
Clone Suffix
105
Sequence Comments
IDP63266.4-452.P(453)..pMCSG68  
Experiment Start Date
May 12, 2016  
Experiment End Date
May 12, 2016  
Person
Gyorgy Babnigg  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG68  
Sequence Start
4  
Sequence End
452  
Reference Sequence Used
CHVHPLPYHS DPSCLFAIIH EAPGAVMLDS GRPIATRGRY DLMSAWPLAE LAPLPDESAN RFFARLREAA GSLGPAQLPA PYELPFAGGL LGYLSYDLGR RIERIGEHAR DDLGLPLASV GLYAWALISD HQAGTSQLVF HPRLDERERQ RLIALFSEEA QGTAGNFKLL GKFRRSISAS DYRQAIRRIQ DYIQAGDCYQ VNYSQRFQAA CSGSPWPAYR ALREACPTPF SGYLRLADGA ILSLSPERFL KLGKGKVETR PIKGTRPRGK TPEEDMALAA SLLASPKDRA ENLMIVDLLR NDIGRSCQPG SVRVPELFAL ESYPNVHHLV SSVTGELAPG KDALDLLEGS FPGGSITGAP KIRAMQIIDE LEPSRRGIYC GSLFYLDVRG EMDSSIAIRT LLVRDGQVSC WGGGGIVADS HWEDEYQETL DKVRVLLETL EGMAGTASP  
PCR Forward Primer
TACTTCCAATCCAATGCCTGTCATGTTCACCCCCTGCCC  
PCR Backward Primer
TTATCCACTTCCAATGTTACGGGGACGCTGTCCCG  
Shipped To Lab
ANL  
Shipped Date
May 27, 2016  
Inserted
May 31, 2016  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2016-05-27 2016-05-27 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay
2016-05-21 2016-05-23 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 1 mM IPTG production