Clone

Target
IDP63266  
Clone Suffix
107
Sequence Comments
IDP63266.2-445.P(453)..pMCSG68  
Experiment Start Date
May 12, 2016  
Experiment End Date
May 12, 2016  
Person
Gyorgy Babnigg  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG68  
Sequence Start
2  
Sequence End
445  
Reference Sequence Used
PSCHVHPLPY HSDPSCLFAI IHEAPGAVML DSGRPIATRG RYDLMSAWPL AELAPLPDES ANRFFARLRE AAGSLGPAQL PAPYELPFAG GLLGYLSYDL GRRIERIGEH ARDDLGLPLA SVGLYAWALI SDHQAGTSQL VFHPRLDERE RQRLIALFSE EAQGTAGNFK LLGKFRRSIS ASDYRQAIRR IQDYIQAGDC YQVNYSQRFQ AACSGSPWPA YRALREACPT PFSGYLRLAD GAILSLSPER FLKLGKGKVE TRPIKGTRPR GKTPEEDMAL AASLLASPKD RAENLMIVDL LRNDIGRSCQ PGSVRVPELF ALESYPNVHH LVSSVTGELA PGKDALDLLE GSFPGGSITG APKIRAMQII DELEPSRRGI YCGSLFYLDV RGEMDSSIAI RTLLVRDGQV SCWGGGGIVA DSHWEDEYQE TLDKVRVLLE TLEG  
PCR Forward Primer
TACTTCCAATCCAATGCCCCCAGTTGTCATGTTCACCCC  
PCR Backward Primer
TTATCCACTTCCAATGTTATCCTTCCAGGGTTTCCAGCAG  
Shipped To Lab
ANL  
Shipped Date
May 27, 2016  
Inserted
May 31, 2016  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2016-05-27 2016-05-27 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay
2014-09-22 2014-09-24 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL success E. coli M9 minimal + semet 0.5 mM IPTG production