Clone

Target
IDP63083  
Clone Suffix
102
Sequence Comments
IDP63083.132-411.P(430)..pMCSG87  
Experiment Start Date
May 12, 2016  
Experiment End Date
May 12, 2016  
Person
Gyorgy Babnigg  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG87  
Sequence Start
132  
Sequence End
411  
Reference Sequence Used
LTYLAYFSFE AKRDGTLKEP TETAKIANIA TQGNTIPMLV ITNIENGNFS ADLTSVILRD ATIQNKFITN ILQTAEKYGM RDIHFDFESV APEDREAYNR FLRNVKTRLP NGYTLSTTLV PKTSSNQKGK FFEAHDYKAQ GQIVDFVVIM TYDWGWQGGP PMAISPIGPV KEVLQYAKSQ MPPQKIMMGQ NLYGFDWKLP FKQGNPPAKA VSSVAAVALA RKYNVPIRYD FTAQAPHFNY FDENGVQHEV WFEDARSIQS KFNLMKEQGI GGISYWKIGL  
PCR Forward Primer
CATCATCACCATCACCATTTAACATACTTAGCTTACTTCAGCTTCGAG  
PCR Backward Primer
ATGGTGATGGTGATGATGCATATGTATATCTCCTTCTTAAAGTTAAAC  
Shipped To Lab
ANL  
Shipped Date
May 27, 2016  
Inserted
May 31, 2016  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2016-05-27 2016-05-27 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay
2016-05-23 2016-05-25 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 1 mM IPTG production