Clone

Target
IDP58950  
Clone Suffix
105
Sequence Comments
IDP58950.48-273.P(273)..pMCSG7  
Experiment Start Date
Jan 04, 2016  
Experiment End Date
Jan 06, 2016  
Person
Jerzy Osipiuk  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG7  
Sequence Start
48  
Sequence End
273  
Reference Sequence Used
TNIEAGKAQA AVSDKMDDQW KERVNPRKKL TPELGEAFAR MYIPQFGSDF QFAIVEGTTD ADLEAGPGHY NDTQLPGERG NFAVAGHRVG KGAPFNDLGN LNVCDAIVVE TRTSWSVYRV MPVDSSGQQR YDEAMGCFTP EQAERITHGD YEHVNGRFIT TPGDVSTISA LPETDVIEAD PGMEGIMTMT TCHPQFSNAE RMIVHAMLTE HFPKNGDNKP AALEEG  
PCR Forward Primer
TACTTCCAATCCAATGCCACCAACATTGAGGCTGGCAAGG  
PCR Backward Primer
TTATCCACTTCCAATGTTAGCCTTCCTCTAACGCTGCTGG  
Shipped To Lab
ANL  
Shipped Date
Feb 08, 2017  
Inserted
Feb 16, 2017  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2016-01-14 2016-01-16 Jerzy Osipiuk Argonne National Laboratory standard expression_ANL success E. coli LB 1 mM IPTG assay
2016-01-20 2016-01-22 Jerzy Osipiuk Argonne National Laboratory standard expression_ANL success E. coli M9 minimal + semet 1 mM IPTG production