Clone

Target
IDP95953  
Clone Suffix
129
Sequence Comments
IDP95953.21-358.P(358)..pMCSG81  
Experiment Start Date
Sep 01, 2017  
Experiment End Date
Dec 04, 2017  
Person
Michael Endres  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG81  
Sequence Start
21  
Sequence End
358  
Reference Sequence Used
LEEISGNRSP SSSKTGSSKA DDLLAAALSA FATIMRPGRQ DMQQLEDLAM PLLQCASTRG KRHAANALAH LEEAPRRLVL ALANEPVEIS APLLLRSPLL RPSDLVDIIA GNGIAHARAI ARRQSDDVLL RGVLRSFADA AIDRTLALQE NLANIDAEPL EKPEIPDLSV ASGPLMRENH SERLVRALQQ PFFTAGALPG CEHLIDTALL IDSQLFRTAL ADALDISFDR ADAIIGKWPD SYLPIALKAL GLSAPECYLV MTAVLGSIET TRDGLRDFVH IYRSIDRAKA MSLVRRWKSD DMSAMLRNKL REMAVSAENE PLDDAANTDA PKPIRSVK  
PCR Forward Primer
GGAGTAAAGATAATGCTGGAGGAAATTTCAGGCAACCG  
PCR Backward Primer
GTGATGGTGATGATGTTTGACCGACCTGATGGGCTTC  
Shipped To Lab
ANL  
Shipped Date
Dec 05, 2017  
Inserted
Dec 05, 2017  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2017-03-04 2017-03-04 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB 1 mM IPTG assay
2017-04-20 2017-04-22 Ruiying Wu Argonne National Laboratory standard expression_ANL success E. coli M9 minimal + semet 0.05% Rhamnose, 0.2% Arabinose, 0.5 mM IPTG production