Clone

Target
IDP00699  
Clone Suffix
2 - The clone is available in BEIR but it is out of stock.
Experiment Start Date
Jul 08, 2008  
Experiment End Date
Jul 08, 2008  
Person
James Winsor  
Lab
Northwestern University  
Protocol ID
NU_default_cloning  
Status
success  
Vector
pMCSG19c  
Sequence Start
1  
Sequence End
496  
Reference Sequence Used
 
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MELLKHLSQR QYIDGEWVES ANKNTRDIIN PYNQEVIFTV SEGTKEDAER AILAARRAFE SGEWSQETAE TRGKKVRAIA DKIKEHREAL ARLETLDTGK TLEESYADMD DIHNVFMYFA GLADKDGGEM IDSPIPDTES KIVKEPVGVV TQITPWNYPL LQASWKIAPA LATGCSLVMK PSEITPLTTI RVFELMEEVG FPKGTINLIL GAGSEVGDVM SGHKEVDLVS FTGGIETGKH IMKNAANNVT NIALELGGKN PNIIFDDADF ELAVDQALNG GYFHAGQVCS AGSRILVQNS IKDKFEQALI DRVKKIKLGN GFDADTEMGP VISTEHRNKI ESYMDVAKAE GATIAVGGKR PDRDDLKDGL FFEPTVITNC DTSMRIVQEE VFGPVVTVEG FETEQEAIQL ANDSIYGLAG AVFSKDIGKA QRVANKLKLG TVWINDFHPY FAQAPWGGYK QSGIGRELGK EGLEEYLVSK HILTNTNPQL VNWFSK  
PCR Forward Primer
 
PCR Backward Primer
 
Shipped To Lab
NU  
Shipped Date
Jun 30, 2008  
Inserted
Aug 08, 2008  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2008-06-16 2008-06-17 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli M9 minimal 1mM IPTG assay
2008-07-28 2008-07-28 James Winsor Northwestern University NU_SelMet_expression success E. coli TB IPTG production
2013-09-30 2013-10-02 Andrei Halavaty Northwestern University NU_default_cloning success E. coli LB IPTG production