Clone

Target

IDP02425  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jan 28, 2009  

Experiment End Date

Jan 29, 2009  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

344  

Reference Sequence Used

MTRTVSMSTA INEAMKISMR RDENVILIGE DVAGGAQVDH LQDDEAWGGV LGVTKGLVQE FGRNRILDTP ISEAGYMGAA MAAAATGLRP IAELMFNDFI GSCLDQVLNQ GAKFRYMFGG KAKVPVTVRT MHGAGFSAAA QHSQSLYALF TSIPGIKVVV PSTPYDAKGL LLAAIEDDDP VIFFEDKTLY NMKGEVPEGY YTIPLGKADI KREGSDVTIV AIGKQVHTAL AAAEQLAKKG LEVEVIDPRS LSPLDEDTIL SSVEKTNRLI VIDEANPRCS IATDIAAIVA DRGFDLLDAP IKRITAPHTP VPFSPPLEKL YLPTPEKVIE TVSEMIGDQS LLHV  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGACTAGAACAGTAAGTATGTC  

PCR Backward Primer

TTATCCACTTCCAATGTTACACATGTAATAAAGATTGGTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Feb 24, 2009