Clone

Target

IDP00720  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

691  

Reference Sequence Used

MLKRLKEKSN DEIVQNTINK RINFIFGVIV FIFAVLVLRL GYLQIAQGSH YKQIIKNDEN ITVNESVPRG RILDRNGKVL VDNASKMAIT YTRGRKTTQS EMLDTAEKLS KLIKMDTKKI TERDKKDFWI QLHPKKAKAM MTKEQAMLAD GSIKQDQYDK QLLSKIGKSQ LDELSSKDLQ VLAIFREMNA GTVLDPQMIK NEDVSEKEYA AVSQQLSKLP GVNTSMDWDR KYPYGDTLRG IFGDVSTPAE GIPKELTEHY LSKGYSRNDR VGKSYLEYQY EDVLRGKKKE MKYTTDKSGK VTSSEVLNPG ARGQDLKLTI DIDLQKEVEA LLDKQIKKLR SQGAKDMDNA MMVVQNPKNG DILALAGKQI NKSGKMTDYD IGTFTSQFAV GSSVKGGTLL AGYQNKAIKV GETMVDEPLH FQGGLTKRSY FNKNGHVTIN DKQALMHSSN VYMFKTALKL AGDPYYSGMA LPSDISSPAQ KLRRGLNQVG LGVKTGIDLP NETRGQIEPL TNNPGNYLDL SIGQYDTYTP LQLSQYVSTI ANDGYRIQPH IGLTIHESTN KDEVGPLKKK INGTVLNKVN NTEKEIKQIQ EGFKMAFNDK DGTGYVSFKD TVVPTAGKTG TAEVFQNGEP RVNSTYIGYA PIDDPKLAFS IVYTNQPVPP PWLTGGDLGR DVINYYFKQL GKDDKNKDKD K  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTTAAAAAGACTAAAAGAAAAATC  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTGTCTTTGTCTTTATTTTTAT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008