Clone

Target
IDP52003  
Clone Suffix
604
Sequence Comments
IDP52003.746-1060.P(1945).K1060InsAA.pMCSG111  
Experiment Start Date
Apr 01, 2020  
Experiment End Date
Apr 01, 2020  
Person
Michael Endres  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG111  
Sequence Start
746  
Sequence End
1060  
Reference Sequence Used
EVRTIKVFTT VDNINLHTQV VDMSMTYGQQ FGPTYLDGAD VTKIKPHNSH EGKTFYVLPN DDTLRVEAFE YYHTTDPSFL GRYMSALNHT KKWKYPQVNG LTSIKWADNN CYLATALLTL QQIELKFNPP ALQDAYYRAR AGEAANFCAL ILAYCNKTVG ELGDVRETMS YLFQHANLDS CKRVLNVVCK TCGQQQTTLK GVEAVMYMGT LSYEQFKKGV QIPCTCGKQA TKYLVQQESP FVMMSAPPAQ YELKHGTFTC ASEYTGNYQC GHYKHITSKE TLYCIDGALL TKSSEYKGPI TDVFYKENSY TTTIK  
PCR Forward Primer
TACTTCCAATCCAATGCCGAAGTGAGGACTATTAAGGTGTTTACAACA  
PCR Backward Primer
TTATCCACTTCCAATGTTATTTTATGGTTGTTGTGTAACTGTTTTCTTTGTAGAAAA  
Shipped To Lab
ANL  
Shipped Date
Apr 06, 2020  
Inserted
Aug 10, 2020  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2020-03-08 2020-03-10 Christine Tesar Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 0.5 mM IPTG production
2020-03-20 2020-03-22 Robert Jedrzejczak Argonne National Laboratory standard expression_ANL success E. coli M9 minimal + semet 0.5 mM IPTG production
2020-04-03 2020-04-03 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay