Clone

Target

IDP00076  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Apr 20, 2009  

Experiment End Date

Apr 20, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

324  

Reference Sequence Used

MQALILEQQD GKTLASVQHL EESQLPAGDV TVDVHWSSLN YKDALAITGK GKIIRHFPMI PGIDFAGTVH ASEDPRFHAG QEVLLTGWGV GENHWGGLAE RARVKGDWLV ALPAGLSSRN AMIIGTAGFT AMLCVMALED AGIRPQDGEV VVTGASGGVG STAVALLHKL GYQVAAVSGR ESTHGYLKSL GANRILSRDE FAESRPLEKQ LWAGAIDTVG DKVLAKVLAQ MNYGGCVAAC GLAGGFALPT TVMPFILRNV RLQGVDSVMT PPARRAEAWA RLVKDLPESF YAQAATEITL ADAPKFADAI INNQVQGRTL VKIK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MQALILEQQD GKTLASVQHL EESQLPAGDV TVDVHWSSLN YKDALAITGK GKIIRHFPMI PGIDFAGTVH ASEDPRFHAG QEVLLTGWGV GENHWGGLAE RARVKGDWLV ALPAGLSSRN AMIIGTAGFT AMLCVMALED AGIRPQDGEV VVTGASGGVG STAVALLHKL GYQVAAVSGR ESTHGYLKSL GANRILSRDE FAESRPLEKQ LWAGAIDTVG DKVLAKVLAQ MNYGGCVAAC GLAGGFALPT TVMPFILRNV RLQGVDSVMT PPARRAEAWA RLVKDLPESF YAQAATEITL ADAPKFADAI INNQVQGRTL VKIK  

PCR Forward Primer

TACTTCCAATCCAATGCGATGCAGGCGTTGATCTTAGAA  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTTATCTTCACCAGCGTAC  

Shipped To Lab

UT  

Shipped Date

Apr 30, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Apr 21, 2009