Clone

Target
IDP00132  
Clone Suffix
1
Experiment Start Date
Apr 20, 2009  
Experiment End Date
Apr 20, 2009  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
618  
Reference Sequence Used
MFSTGSGELC DSSAARAGGS MIHPILAKVT QNLTERSREA RAAFIARSQA QEKAGKGRTS LSCGNLAHAV AASCSTEKKA ILDFTRSNVA IVTSYNDMLS AHQPYQHYPD RIKMVLAEYG HTAQVAGGVP AMCDGVTQGQ PGMDMSLFSR DLIAQATALS LSHNVFDATL LLGICDKIAP GQLMGALSYA HLPTAFMPAG LMATGISNEE KVDIRQKYAA GEVGKDALLN MECQAYHAPG TCTFYGTANT NQLVFEAMGL MLPGSAFVHP HSALRQALND DATVKIASMT QGSAQYRPLY QVVTEKSLLN GIVALLASGG STNHTIHMLA VARAAGILLT WQDISDLSDV VPLLAKVYPN GPADMNAFQQ AGGVPALMKR LHESKLLHSD VTPVFGEFRD QLTLPELVDG ELCWKACEGT RDAQVIAASG ECFQTTGGTK MLNGNLGRAV IKVSAVKEEQ RIIEAPAVVF QCQHQVEAAY QRGELNKDCI VVVTHNGPAA NGMPELHKLM PILGNVQKAG FKVALVTDGR LSGASGKIPS AIHVSPEAVR GGAIGLVRDG DLLRLDCTTG TLENLTDMSH RQALALDTER DQQMWGRELF KVMRQAVSSA EQGASFIV  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MFSTGSGELC DSSAARAGGS MIHPILAKVT QNLTERSREA RAAFIARSQA QEKAGKGRTS LSCGNLAHAV AASCSTEKKA ILDFTRSNVA IVTSYNDMLS AHQPYQHYPD RIKMVLAEYG HTAQVAGGVP AMCDGVTQGQ PGMDMSLFSR DLIAQATALS LSHNVFDATL LLGICDKIAP GQLMGALSYA HLPTAFMPAG LMATGISNEE KVDIRQKYAA GEVGKDALLN MECQAYHAPG TCTFYGTANT NQLVFEAMGL MLPGSAFVHP HSALRQALND DATVKIASMT QGSAQYRPLY QVVTEKSLLN GIVALLASGG STNHTIHMLA VARAAGILLT WQDISDLSDV VPLLAKVYPN GPADMNAFQQ AGGVPALMKR LHESKLLHSD VTPVFGEFRD QLTLPELVDG ELCWKACEGT RDAQVIAASG ECFQTTGGTK MLNGNLGRAV IKVSAVKEEQ RIIEAPAVVF QCQHQVEAAY QRGELNKDCI VVVTHNGPAA NGMPELHKLM PILGNVQKAG FKVALVTDGR LSGASGKIPS AIHVSPEAVR GGAIGLVRDG DLLRLDCTTG TLENLTDMSH RQALALDTER DQQMWGRELF KVMRQAVSSA EQGASFIV  
PCR Forward Primer
TACTTCCAATCCAATGCGATGTTCAGTACAGGAAGTGGT  
PCR Backward Primer
TTATCCACTTCCAATGTTAAACAATAAAGCTCGCCCC  
Shipped To Lab
ANL  
Shipped Date
Jul 20, 2010  
Inserted
Apr 21, 2009