Clone

Target

IDP00133  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Apr 20, 2009  

Experiment End Date

Apr 20, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

548  

Reference Sequence Used

MAMHPRAGQK ARQEDLHNIP ALVANYYLLQ PEAGNTAHKV EFGTSGHRGT ADKTTFNEHH ILAIAQAVAD VRHEHGVTGP IFIGKDTHAL SEPAFSSVLE VLIANGIEVI VQENNGYTPT PGISHAILTY NLKHADKADG IVITPSHNPP QDGGIKYNPP HGGPAEGELT QAIEDRANAY ISQQLAGVKR MPIALAKQSE LLKQVDLVKP YVDDLVNVVD MAAIQKAKLK IGVDPLGGSG IDYWRQIGNA YQLDLTLVSE AIDPSFQFMS LDKDGVIRMD CSSPYAMAGL LALKDEYDLA FGNDPDYDRH GIVTPKGLMN PNHFLAVCID YLYRHRQGWA GHVAVGKTLV SSAMIDKVVA DLGRELCEVP VGFKWFVDGL YSGRFGFGGE ESAGASFLRQ DGTPWSTDKD GIILCLLAAE ITAVTGKNPQ QYYDELAAKH GDFYYSRIQA VANGPQKNVL KKLSPEMVSA QTLAGDAITA RLTHAPGNGA AIGGLKVTTD YGWFAARPSG TEDIYKIYCE SFKGAEHLKQ IESEAQQIVN QVFKDAGL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MAMHPRAGQK ARQEDLHNIP ALVANYYLLQ PEAGNTAHKV EFGTSGHRGT ADKTTFNEHH ILAIAQAVAD VRHEHGVTGP IFIGKDTHAL SEPAFSSVLE VLIANGIEVI VQENNGYTPT PGISHAILTY NLKHADKADG IVITPSHNPP QDGGIKYNPP HGGPAEGELT QAIEDRANAY ISQQLAGVKR MPIALAKQSE LLKQVDLVKP YVDDLVNVVD MAAIQKAKLK IGVDPLGGSG IDYWRQIGNA YQLDLTLVSE AIDPSFQFMS LDKDGVIRMD CSSPYAMAGL LALKDEYDLA FGNDPDYDRH GIVTPKGLMN PNHFLAVCID YLYRHRQGWA GHVAVGKTLV SSAMIDKVVA DLGRELCEVP VGFKWFVDGL YSGRFGFGGE ESAGASFLRQ DGTPWSTDKD GIILCLLAAE ITAVTGKNPQ QYYDELAAKH GDFYYSRIQA VANGPQKNVL KKLSPEMVSA QTLAGDAITA RLTHAPGNGA AIGGLKVTTD YGWFAARPSG TEDIYKIYCE SFKGAEHLKQ IESEAQQIVN QVFKTRVY  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGCTATGCACCCTCGTG  

PCR Backward Primer

TTATCCACTTCCAATGTTATAAACCCGCGTCTTTAAAC  

Shipped To Lab

ANL  

Shipped Date

Jul 20, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Apr 21, 2009