Clone

Target

IDP98450  

Clone Suffix

105

Sequence Comments

IDP98450.1-862.F..pMCSG53  

Experiment Start Date

Dec 04, 2020  

Experiment End Date

Dec 04, 2020  

Person

Michael Endres  

Lab

Argonne National Laboratory  

Protocol ID

standard cloning_ANL  

Status

failed  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

862  

Reference Sequence Used

MFRIAMTDFI DILRSARGYN NQIVHVERLP ARAARYAEPS APLPPPLAAA LAARGISRLY THQAAALDAA RAGLHVGIVT ATASGKTLCY QLPTIEATLS DPAARALFLF PTKALAADQL RALYTLLDAL KAAGGPALMA ATLDGDTPRA TRDRVRAGAS IILSNPDLLH RTLLPDHARW QSVLAQLRFV VLDEAHTYRG VFGTHVALIM RRLRRLCAHY GSAPQFICCS ATSANPQHHL AALIGDEVRV IDDDGAPQGE RTFLFWNPPV IDNQRVPTWM RTLLPENGQR RSTNVETANV LATLVRAGVK TLAFARTRRS AELVLRYARE TLDNGDSVEN GHNPSMPLAE QIAAYRAGYT VEDRRRLEQA FARGDLRGLV STNALELGVD IGGVDAVVIG GFPGTIASAW QQAGRAGRAQ GKSLAALVAQ DDPLDQFYMR HPAEFFCRPH EHARIALDNP YILRDQLRCA AAELPLHDAD DLWFGATFAK LRDWLLQRGE VTTRPDGRAA YAGDPRPAAH VNIRSADGNP IDLIDIDTGR RIEQIAATRA PFEVFPGAVY MHQGNTYVVE ALTSRQALAR RAQIEYYTRT IDETEIAVRQ VRQQRQVGPT TLALGVVDVT RQVVGYKRKK HYTDDVISEH ELTMPPQTFR TIAVWWTISD SACRQIAQVC DSVLDALHAM EHAVIGLLPL FAQCDRADIG GLSTDAHPDT GAATIFVYDG VPGGVGIAEV GYEQAAQWWE QTWRLLVDCP CSEGCPACIQ SPKCGNANQH LSKIGAATLA ALLTGHAPPL PDAFGRHATS AAFIEEMQQQ LARARSEPPG VRRTAVLVAL RYRIATARAS ITDDTGRATL AMIEAEANAL QR  

Reference Sequence Variance

 

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCCTTGTTCCGAATCGCTATGACCGATTTTATT  

PCR Backward Primer

TTATCCACTTCCAATGTTATCGTTGCAGCGCATTCGCTTC  

Shipped To Lab

ANL  

Shipped Date

Dec 04, 2020  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Dec 07, 2020