Clone

Target
IDP01923  
Clone Suffix
1 - The clone is available in BEIR but it is out of stock.
Experiment Start Date
Apr 20, 2009  
Experiment End Date
Apr 20, 2009  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
714  
Reference Sequence Used
MTKPLNTTQA VIEWVNNTRR YATRLDDEAD ALLAQLTLAA ADESALNAAC ASHGCVGLYG YAQSAKAHLL TTLCGNENGK LEIITPDRDY DYFSHINPGH APANMAIRFT RDIFSNESGW PLRLRLISEA ELVQIFIAWT SSSPVCRQVE KSIITSRLEK WQSLRQPQPV PGVTAEEVAT IASFWRSCLP SARQHIDDAT WQHFASLLPA LDLTTRAHAW ALLWGEQPEI TQQWLALAHM LQQTGHAGEL AAPLSLLVDH FGLPAENFLT QMALTANDTQ SDVVVHPVKE GRLLNAVSLS LDSLALLTRE LVLSVENNVL DNVDLLDIPV APDSHPHPLW RAKLGWMLAH YRQQVQPDVL VICNALASRS QTSTAAHHLL EWVNATQPQH ESALPGVVWA ITPQDARFAT QQNLDEAVQQ LMGKPGVHWG TLQALDKHSM QRLVEWLSQA TSAPQRQARL QALREQLRGR VRDLLPMFDD ARLPVETVIR RLQAQAARHG DLLAGLLPPV QNFEALLSTR QSREEQVCGL FNDAIDLFAD EPTRASASEG HETGYQAHKM WINHLRQWAH CRDNAQRLGL EPQMLNAVAE ILITASYRLG LPQQLQKTMQ REEVSGAQLH AIIGNFIAWL GYANIEEAQR PASRVQKGAA IFAATPRSTM LRLTKLDEQP VHAASRYVYD WLVALYTLAN ENAGYRHPQD VTDVDRAQLI ALIA  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MTKPLNTTQA VIEWVNNTRR YATRLDDEAD ALLAQLTLAA ADESALNAAC ASHGCVGLYG YAQSAKAHLL TTLCGNENGK LEIITPDRDY DYFSHINPGH APANMAIRFT RDIFSNESGW PLRLRLISEA ELVQIFIAWT SSSPVCRQVE KSIITSRLEK WQSLRQPQPV PGVTAEEVAT IASFWRSCLP SARQHIDDAT WQHFASLLPA LDLTTRAHAW ALLWGEQPEI TQQWLALAHM LQQTGHAGEL AAPLSLLVDH FGLPAENFLT QMALTANDTQ SDVVVHPVKE GRLLNAVSLS LDSLALLTRE LVLSVENNVL DNVDLLDIPV APDSHPHPLW RAKLGWMLAH YRQQVQPDVL VICNALASRS QTSTAAHHLL EWVNATQPQH ESALPGVVWA ITPQDARFAT QQNLDEAVQQ LMGKPGVHWG TLQALDKHSM QRLVEWLSQA TSAPQRQARL QALREQLRGR VRDLLPMFDD ARLPVETVIR RLQAQAARHG DLLAGLLPPV QNFEALLSTR QSREEQVCGL FNDAIDLFAD EPTRASASEG HETGYQAHKM WINHLRQWAH CRDNAQRLGL EPQMLNAVAE ILITASYRLG LPQQLQKTMQ REEVSGAQLH AIIGNFIAWL GYANIEEAQR PASRVQKGAA IFAATPRSTM LRLTKLDEQP VHAASRYVYD WLVALYTLAN ENAGYRHPQD VTDVDRAQLI ALIA  
PCR Forward Primer
TACTTCCAATCCAATGCGATGACAAAACCGTTAAACACCA  
PCR Backward Primer
TTATCCACTTCCAATGTCAGGCTATCAGGGCAATTAA  
Shipped To Lab
UT  
Shipped Date
Apr 30, 2009  
Inserted
Apr 21, 2009  

Related Expressions

                                             
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2009-04-29 2009-04-30 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2012-12-20 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2012-12-20 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2012-12-20 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2014-02-12 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2014-02-12 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2014-02-12 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2014-02-12 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2014-02-12 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2014-02-12 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2012-12-20 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2012-12-20 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2014-02-12 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2014-02-12 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2012-12-20 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2012-12-20 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2012-12-20 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2012-12-20 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2012-12-20 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2014-02-12 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2014-02-12 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2014-02-12 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production
2014-02-12 Veronica Yim University of Calgary Expression_protocol_Toronto success E. coli Studier w/o lactose none production