Clone

Target
IDP52003  
Clone Suffix
667
Sequence Comments
IDP52003.678-1060.P(1945)..pMCSG53  
Experiment Start Date
Oct 27, 2021  
Experiment End Date
Oct 27, 2021  
Person
Michael Endres  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
failed  
Vector
pMCSG53  
Sequence Start
678  
Sequence End
1060  
Reference Sequence Used
TPEEHFIETI SLAGSYKDWS YSGQSTQLGI EFLKRGDKSV YYTSNPTTFH LDGEVITFDN LKTLLSLREV RTIKVFTTVD NINLHTQVVD MSMTYGQQFG PTYLDGADVT KIKPHNSHEG KTFYVLPNDD TLRVEAFEYY HTTDPSFLGR YMSALNHTKK WKYPQVNGLT SIKWADNNCY LATALLTLQQ IELKFNPPAL QDAYYRARAG EAANFCALIL AYCNKTVGEL GDVRETMSYL FQHANLDSCK RVLNVVCKTC GQQQTTLKGV EAVMYMGTLS YEQFKKGVQI PCTCGKQATK YLVQQESPFV MMSAPPAQYE LKHGTFTCAS EYTGNYQCGH YKHITSKETL YCIDGALLTK SSEYKGPITD VFYKENSYTT TIK  
PCR Forward Primer
TACTTCCAATCCAATGCCACACCTGAAGAACATTTTATTGAAACCATCTCA  
PCR Backward Primer
TTATCCACTTCCAATGTTATTTTATGGTTGTTGTGTAACTGTTTTCTTTGTAGAAAA  
Shipped To Lab
ANL  
Shipped Date
Nov 05, 2021  
Inserted
Nov 01, 2021  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2021-10-31 2021-11-02 Christine Tesar Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 0.5 mM IPTG production
2021-12-08 2021-12-10 Christine Tesar Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 0.5 mM IPTG production
2021-11-05 2021-11-05 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB 1 mM IPTG assay